Molecular analysis of multidrug-resistant E. coli in pediatric UTIs: findings from a Nigerian Hospital

Journal article


Wilkie, E.D., Ezeani, C.V., Olasehinde, D.O., Oyedemi, O.T., Alao, J.O., Oluduro, A.O. and Oyetunde, O.B. 2024. Molecular analysis of multidrug-resistant E. coli in pediatric UTIs: findings from a Nigerian Hospital . The Journal of Infection in Developing Countries . 18 (2), pp. 1-8. https://doi.org/10.3855/jidc.18520
AuthorsWilkie, E.D., Ezeani, C.V., Olasehinde, D.O., Oyedemi, O.T., Alao, J.O., Oluduro, A.O. and Oyetunde, O.B.
Abstract

Introduction: This study aimed to isolate and characterize antibiotic-resistant Escherichia coli from urine samples of children at the Mother and Child Hospital in Ondo State, Nigeria, assessing antibiogram profiling and resistance genes.

Methodology: Three hundred urine samples (158 females, 142 males), aged 3-5 years, were collected, transported on ice, and analyzed bacteriologically. E. coli and Gram-negative bacteria were isolated using Eosin Methylene Blue agar and identified through colony morphology and biochemical tests. Antibiotic susceptibility was determined via Kirby Bauer's disc diffusion, and resistance genes were detected using Polymerase Chain Reaction (PCR).

Results: Of the 300 samples, 40 (13.3%) yielded E. coli with varying antibiotic resistance profiles. The highest resistance was against Amoxicillin-clavulanate (87.5%) followed by Ceftriaxone (80%). Susceptibility was observed to Nitrofurantoin, Erythromycin, and Chloramphenicol. Multiple resistance patterns against 3-4 antibiotic classes were recorded, with 12 distinct patterns observed. Eight isolates harbored blaCTX-M gene, while five carried the aac3-IV gene.

Conclusions: The study concluded a high occurrence of E. coli infection and multiple antibiotic resistance in the region. The presence of resistance genes suggests significant economic and health implications, emphasizing prudent antibiotic use under physician guidance to mitigate multiple antibiotic resistance.

Keywordsantibiotic-resistant Escherichia coli; urine samples ; e-coli ; gene
Year2024
JournalThe Journal of Infection in Developing Countries
Journal citation18 (2), pp. 1-8
PublisherThe Journal of Infection in Developing Countries , Italy
ISSN1972-2680
Digital Object Identifier (DOI)https://doi.org/10.3855/jidc.18520
Web address (URL)https://jidc.org/index.php/journal/article/view/38484349
Publisher's version
License
File Access Level
Open
Output statusPublished
Publication dates
Online29 Feb 2024
Publication process dates
Deposited03 Apr 2024
Permalink -

https://repository.derby.ac.uk/item/q577x/molecular-analysis-of-multidrug-resistant-e-coli-in-pediatric-utis-findings-from-a-nigerian-hospital

Download files


Publisher's version
jidc-018-0251 (1).pdf
License: CC BY 4.0
File access level: Open

  • 0
    total views
  • 0
    total downloads
  • 0
    views this month
  • 0
    downloads this month

Export as