Polymerase chain reaction detection of avipox and avian papillomavirus in naturally infected wild birds: comparisons of blood, swab and tissue samples

Journal article


Williams, Richard, Escudero Duch, Clara, Pérez-Tris, Javier and Benítez, Laura 2014. Polymerase chain reaction detection of avipox and avian papillomavirus in naturally infected wild birds: comparisons of blood, swab and tissue samples. Avian Pathology. 43 (2), pp. 130-134. https://doi.org/10.1080/03079457.2014.886326
AuthorsWilliams, Richard, Escudero Duch, Clara, Pérez-Tris, Javier and Benítez, Laura
Abstract

Avian poxvirus (avipox) is widely reported from avian species, causing cutaneous or mucosal lesions. Mortality rates of up to 100% are recorded in some hosts. Three major avipox clades are recognized. Several diagnostic techniques have been reported, with molecular techniques used only recently. Avipox has been reported from 278 different avian species, but only 111 of these involved sequence and/or strain identification. Collecting samples from wild birds is challenging as only few wild bird individuals or species may be symptomatic. Also, sampling regimes are tightly regulated and the most efficient sampling method, whole bird collection, is ethically challenging. In this study, three alternative sampling techniques (blood, cutaneous swabs and tissue biopsies) from symptomatic wild birds were examined. Polymerase chain reaction was used to detect avipoxvirus and avian papillomavirus (which also induces cutaneous lesions in birds). Four out of 14 tissue samples were positive but all 29 blood samples and 22 swab samples were negative for papillomavirus. All 29 blood samples were negative but 6/22 swabs and 9/14 tissue samples were avipox-positive. The difference between the numbers of positives generated from tissue samples and from swabs was not significant. The difference in the avipox-positive specimens in paired swab (4/6) and tissue samples (6/6) was also not significant. These results therefore do not show the superiority of swab or tissue samples over each other. However, both swab (6/22) and tissue (8/9) samples yielded significantly more avipox-positive cases than blood samples, which are therefore not recommended for sampling these viruses.

KeywordsPOXVIRUS INFECTION; VIRUS; PCR; IDENTIFICATION; DIAGNOSIS
Year2014
JournalAvian Pathology
Journal citation43 (2), pp. 130-134
PublisherTaylor and Francis Group
ISSN0307-9457
1465-3338
Digital Object Identifier (DOI)https://doi.org/10.1080/03079457.2014.886326
Web address (URL)http://hdl.handle.net/10545/623505
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
hdl:10545/623505
Publication dates04 Mar 2014
Publication process dates
Deposited19 Feb 2019, 12:09
Accepted26 Dec 2013
Rights

Attribution-NonCommercial-ShareAlike 3.0 United States

ContributorsDepartment of Zoology and Physical Anthropology, Faculty of Biological Sciences, Universidad Complutense de Madrid, Spain, Natural Sciences, Saint Louis University, Madrid, Spain and Department of Microbiology, Faculty of Biological Sciences, Universidad Complutense de Madrid, Madrid, Spain
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