Optimization of water content for the cryopreservation of allium sativum In vitro cultures by encapsulation-dehydration

Journal article


Lynch, Paul, Souch, Graham, Zamecnik, Jiri and Harding, Keith 2016. Optimization of water content for the cryopreservation of allium sativum In vitro cultures by encapsulation-dehydration. CryoLetters.
AuthorsLynch, Paul, Souch, Graham, Zamecnik, Jiri and Harding, Keith
Abstract

BACKGROUND: There is a general requirement to determine and correlate water content to viability for the standardization of conservation protocols to facilitate effective cryostorage of plant germplasm. OBJECTIVE: This study examined water content as a critical factor to optimize the cryostorage of Allium sativum. MATERIALS AND METHODS: Stem discs were excised from post-harvest, stored bulbs prior to cryopreservation by encapsulation-dehydration and water content was determined gravimetrically. RESULTS: Survival of cryopreserved stem discs was 42.5%, with 22.5% exhibiting shoot regrowth following 6 h desiccation. Gravimetric data demonstrated a correlation between water content corresponding with survival / regrowth from desiccated, cryopreserved stem discs. For encapsulated stem discs a 25% residual moisture and corresponding water content of 0.36 g H2O g-1 d.wt correlated with maximal survival following ~6.5 h of desiccation. CONCLUSION: The data concurs with the literature suggesting the formation of a stable vitrified state and a ‘window’ for optimal survival and regrowth that is between 6 – 10 h desiccation. Further studies using differential scanning calorimetry (DSC) are suggested to substantiate these findings

KeywordsCryopreservation; Garlic; Survival; Vitrification
Year2016
JournalCryoLetters
PublisherCryoLetters
ISSN0143-2044
Web address (URL)http://hdl.handle.net/10545/620697
hdl:10545/620697
Publication datesOct 2016
Publication process dates
Deposited07 Nov 2016, 11:41
ContributorsUniversity of Derby
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File Access Level
Open
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File Access Level
Open
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https://repository.derby.ac.uk/item/92x6z/optimization-of-water-content-for-the-cryopreservation-of-allium-sativum-in-vitro-cultures-by-encapsulation-dehydration

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