Cryopreservation of quince (Cydonia oblonga mill.)

Journal article


Lynch, Paul, Siddika, Ayesha, Mehra, Aradhana, Benelli, Carla and Lambardi, Maurizo 2014. Cryopreservation of quince (Cydonia oblonga mill.). CryoLetters.
AuthorsLynch, Paul, Siddika, Ayesha, Mehra, Aradhana, Benelli, Carla and Lambardi, Maurizo
Abstract

BACKGROUND: Quince (Cydonia oblonga Mill.) has great potential for utilisation in pharmaceutical and food industries. OBJECTIVE: The study was to develop an efficient cryopreservation approachfor quince. METHODS: Factors on the survival and regrowth such as cold acclimation, explant type and recovery media composition were assessed. The effectiveness of the resultant protocols for a number of quince cultivars was determined. RESULTS and CONCLUSION: Quince shoot tips and nodal sections are successfully cryopreserved. Sustained regrowth of quince ‘Angers A’ was observed after encapsulation-osmoprotection/dehydration, encapsulation-dehydration and PVS2 vitrification. The highest regrowth rate (80%) was obtained from explants excised from cold hardened shoots and cryopreserved using encapsulation-osmoprotection/dehydration and vitrification protocols. The optimised vitrification protocol in combination with shoot cold hardening and a MS recovery mediumwithout activated charcoal and auxin resulted in satisfactory regrowth of shoots from six quince cultivars. The morphology of acclimatised plants derived from cryopreserved shoots was comparablewith non-cryopreserved plants.

BACKGROUND: Quince (Cydonia oblonga Mill.) has great potential for utilisation in pharmaceutical and food industries. OBJECTIVE: The study was to develop an efficient cryopreservation approachfor quince. METHODS: Factors on the survival and regrowth such as cold acclimation, explant type and recovery media composition were assessed. The effectiveness of the resultant protocols for a number of quince cultivars was determined. RESULTS and CONCLUSION: Quince shoot tips and nodal sections are successfully cryopreserved. Sustained regrowth of quince ‘Angers A’ was observed after encapsulation-osmoprotection/dehydration, encapsulation-dehydration and PVS2 vitrification.
The highest regrowth rate (80%) was obtained from explants excised from cold hardened shoots and cryopreserved using encapsulation-osmoprotection/dehydration and vitrification protocols. The optimised vitrification protocol in combination with shoot cold hardening and a MS recovery mediumwithout activated charcoal and auxin resulted in satisfactory regrowth of shoots from six quince cultivars. The morphology of acclimatised plants derived from cryopreserved shoots was comparablewith non-cryopreserved plants.

KeywordsQuince; Shoot tips; Nodal sections; Cold hardening; Recovery medium
Year2014
JournalCryoLetters
PublisherCryoLetters
ISSN0143-2044
Web address (URL)http://hdl.handle.net/10545/620698
hdl:10545/620698
Publication dates2014
Publication process dates
Deposited07 Nov 2016, 11:45
ContributorsUniversity of Derby
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File Access Level
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