Development and in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold

Journal article


Pitrolino, K. A., Felfel, R. M., Macri Pellizzeri, L, McLaren, J., Popov, A. A., Sottile, V., Scotchford, C. A., Scammell, B. E., Roberts, G. A. F. and Grant, D. M. 2022. Development and in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold. Carbohydrate Polymers. 282, pp. 1-13. https://doi.org/10.1016/j.carbpol.2022.119126
AuthorsPitrolino, K. A., Felfel, R. M., Macri Pellizzeri, L, McLaren, J., Popov, A. A., Sottile, V., Scotchford, C. A., Scammell, B. E., Roberts, G. A. F. and Grant, D. M.
Abstract

An innovative approach was developed to engineer a multi-layered chitosan scaffold for osteochondral defect repair. A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160–275 μm). Incorporation of 70 wt% nano-hydroxyapatite (nHA) provided additional strength to the bone-like layer. The scaffold showed instantaneous mechanical recovery under compressive loading and did not delaminate under tensile loading. The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer. Osteogenic and chondrogenic differentiation of MSCs preferentially occurred in selected layers of the scaffold in vitro, driven by the distinct pore gradient and material composition. This scaffold is a suitable candidate for minimally invasive arthroscopic delivery in the clinic with potential to regenerate damaged cartilage and bone.

KeywordsOsteochondral Repair; Tissue Engineering Scaffold; Regenerative Medicine; Chitosan Scaffold; Nano hydroxyapatite; Mesenchymal Stem Cells; Graded porosity
Year2022
JournalCarbohydrate Polymers
Journal citation282, pp. 1-13
PublisherElseiver
ISSN0144-8617
Digital Object Identifier (DOI)https://doi.org/10.1016/j.carbpol.2022.119126
Web address (URL)https://www.sciencedirect.com/science/article/pii/S0144861722000303?via%3Dihub
FunderEngineering and Physical Sciences Research Council
Publisher's version
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Open
Output statusPublished
Publication dates
Online12 Jan 2022
Publication process dates
Accepted07 Jan 2022
Completed15 Apr 2022
Deposited11 Apr 2022
Supplemental file
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File Access Level
Open
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