In vitro degradation of a chitosan-based osteochondral construct points to a transient effect on cellular viability

Journal article


Pitrolino, K, Felfel, R., Roberts, G., Scotchford, C., Grant, D. and Sottile, V. 2024. In vitro degradation of a chitosan-based osteochondral construct points to a transient effect on cellular viability. Biomedical Materials . 19 (5), pp. 1-16. https://doi.org/10.1088/1748-605X/ad6547
AuthorsPitrolino, K, Felfel, R., Roberts, G., Scotchford, C., Grant, D. and Sottile, V.
Abstract

Bioresorbable chitosan scaffolds have shown potential for osteochondral repair applications. The in vivo degradation of chitosan, mediated by lysozyme and releasing glucosamine, enables progressive replacement by ingrowing tissue. Here the degradation process of a chitosan-nHA based bioresorbable scaffold was investigated for mass loss, mechanical properties and degradation products released from the scaffold when subjected to clinically relevant enzyme concentrations. The scaffold showed accelerated mass loss during the early stages of degradation but without substantial reduction in mechanical strength or structure deterioration. Although not cytotoxic, the medium in which the scaffold was degraded for over 2 weeks showed a transient decrease in mesenchymal stem cell viability, and the main degradation product (glucosamine) demonstrated a possible adverse effect on viability when added at its peak concentration. This study has implications for the design and biomedical application of chitosan scaffolds, underlining the importance of modelling degradation products to determine suitability for clinical translation.

KeywordsBioresorbable chitosan scaffolds; in vivo degradation; clinical translation
Year2024
JournalBiomedical Materials
Journal citation19 (5), pp. 1-16
PublisherIOP Publishing Ltd
ISSN 1748-605X
Digital Object Identifier (DOI)https://doi.org/10.1088/1748-605X/ad6547
Web address (URL)https://iopscience.iop.org/article/10.1088/1748-605X/ad6547/meta
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Open
Output statusPublished
Publication dates
Online06 Aug 2024
Publication process dates
Deposited19 Aug 2024
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