Antioxidative effects of flavonoids and their metabolites against hypoxia/reoxygenation-induced oxidative stress in a human first trimester trophoblast cell line.
Journal article
| Authors | Ebegboni, Vernon J, Dickenson John M and Sivasubramaniam, Shiva |
|---|---|
| Abstract | This study aimed to investigate the cytoprotective effects of flavonoids, their metabolites alone or in combination against hypoxia/reoxygenation induced oxidative stress in the transformed human first trimester trophoblast cell line (HTR-8/SVneo). Oxidative stress was achieved with hypoxia followed by reoxygenation and the following assays were performed: MTT, CellTox™ Green Cytotoxicity, CellTiter-Glo®, NADP/NADPH-Glo™, ROS-Glo™/H2O2, GSH/GSSG-Glo™ and Caspase-Glo® 3/7 assays. HTR-8/SVneo cells, pre-treated for 24 h with flavonoids or their metabolites were protected significantly from oxidative stress. Flavonoids were associated with ROS modulation, reducing the generation of superoxide/hydrogen peroxide. The activities of caspases 3/7 were also significantly reduced significantly in HTR-8/SVneo cells pre-treated with flavonoids. This study has shown for the first time that 24 h pre-treatment with flavonoids, their metabolites alone or in combination, protected against HR-induced oxidative stress in the trophoblast cell line. These data indicate that dietary flavonoids may be beneficial to placental health and invasion during early gestation. |
This study aimed to investigate the cytoprotective effects of flavonoids, their metabolites alone or in combination against hypoxia/reoxygenation induced oxidative stress in the transformed human first trimester trophoblast cell line (HTR-8/SVneo). Oxidative stress was achieved with hypoxia followed by reoxygenation and the following assays were performed: MTT, CellTox™ Green Cytotoxicity, CellTiter-Glo®, NADP/NADPH-Glo™, ROS-Glo™/H2O2, GSH/GSSG-Glo™ and Caspase-Glo® 3/7 assays. HTR-8/SVneo cells, pre-treated for 24 h with flavonoids or their metabolites were protected significantly from oxidative stress. Flavonoids were associated with ROS modulation, reducing the generation of superoxide/hydrogen peroxide. The activities of caspases 3/7 were also significantly reduced significantly in HTR-8/SVneo cells pre-treated with flavonoids. This study has shown for the first time that 24 h pre-treatment with flavonoids, their metabolites alone or in combination, protected against HR-induced oxidative stress in the trophoblast cell line. These data indicate that dietary flavonoids may be beneficial to placental health and invasion during early gestation. | |
| Keywords | Trophoblast; Antioxidant; Flavonoids; Quercertin; hypoxia/reoxygenation |
| Year | 2018 |
| Journal | Food Chemistry |
| Publisher | Elsevier |
| ISSN | 0308-8146 |
| Digital Object Identifier (DOI) | https://doi.org/10.1016/j.foodchem.2018.08.036 |
| Web address (URL) | http://hdl.handle.net/10545/623206 |
| hdl:10545/623206 | |
| Publication dates | 09 Aug 2018 |
| Publication process dates | |
| Deposited | 08 Dec 2018, 12:27 |
| Accepted | 08 Aug 2018 |
| Contributors | Nottingham Trent University |
| File | File Access Level Open |
| File |
https://repository.derby.ac.uk/item/948yw/antioxidative-effects-of-flavonoids-and-their-metabolites-against-hypoxia-reoxygenation-induced-oxidative-stress-in-a-human-first-trimester-trophoblast-cell-line
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