Exopolysaccharides from Lactobacillus acidophilus modulates the antioxidant status of 1,2–dimethyl hydrazine-induced colon cancer rat model

Journal article


Venkataraman Deepak, Arputha Sundar, William, Ram Kumar Pandian, Sureshbabu, Sivasubramaniam, Shiva D., Hariharan, Nellaiah and Sundar, Krishnan 2021. Exopolysaccharides from Lactobacillus acidophilus modulates the antioxidant status of 1,2–dimethyl hydrazine-induced colon cancer rat model. 3 Biotech. 11 (5), pp. 1-9. https://doi.org/10.1007/s13205-021-02784-x
AuthorsVenkataraman Deepak, Arputha Sundar, William, Ram Kumar Pandian, Sureshbabu, Sivasubramaniam, Shiva D., Hariharan, Nellaiah and Sundar, Krishnan
Abstract

The aim of the current study is to ascertain the anticancer activity of exopolysaccharides (EPS) from probiotic Lactobacillus acidophilus in the 1, 2 – dimethyl hydrazine (DMH) induced colon cancer rat model and to determine the antioxidant status. Rats were divided into five groups of six animals each. Group I served as control, group II served as cancer control (DMH alone administered), group III as standard drug control [Fluorouracil (5-FU) along with DMH} and group IV and V received EPS in two doses (200 mg/kg body weight and 400 mg/kg body weight along with DMH). EPS administration was found to reduce the number of polyps formed (Group IV - 8.25±1.258 & Group V - 8.50±1.732 vs Group II - 14.50±2.380) and to increase the levels of antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and antioxidants like vitamin C (Vit. C), reduced glutathione (GSH) which was found to be reduced in colon cancer control rats. The status of lipid peroxidation (LPO) was also evaluated. All the values which were affected by the supplementation of DMH were brought to near normal levels by the treatment with EPS. The well preserved histology of colon and the biochemical evaluation also show that EPS could be a potential agent for the prevention and treatment of colon cancer.

KeywordsBiotechnology; Agricultural and Biological Sciences (miscellaneous); Environmental Science (miscellaneous)
Year2021
Journal3 Biotech
Journal citation11 (5), pp. 1-9
PublisherSpringer Science and Business Media LLC
ISSN2190-572X
2190-5738
Digital Object Identifier (DOI)https://doi.org/10.1007/s13205-021-02784-x
Web address (URL)https://link.springer.com/article/10.1007%2Fs13205-021-02784-x#Sec2
https://www.springer.com/tdm
hdl:10545/625803
Output statusPublished
Publication dates19 Apr 2021
Publication process dates
Deposited28 May 2021, 11:33
Accepted08 Apr 2021
ContributorsUniversity of Derby
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