Doxorubicin resistant choriocarcinoma cell line derived spheroidal cells exhibit stem cell markers but reduced invasion
Journal article
Authors | Sivasubramaniam, S. and Venkataraman, D. |
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Abstract | Cell cycle-specifc cancer chemotherapy is based on the ability of a drug to halt, minimise or destroy rapidly dividing cells. However, their efcacy is limited by the emergence of a self-renewing cell pool called “cancer stem cells” (CSC). Choriocarcinoma is a tumour of trophoblastic tissue. We, in this study, analysed whether spheroids generated from doxorubicintreated and non-treated choriocarcinoma cell lines exhibit markers of stem cells. Two choriocarcinoma cell lines, namely JEG-3 and BeWo, were used in this study. Spheroids were generated from doxorubicin-treated cells and the non-treated cells under non-adherent condition, followed by analysis of stem-cell markers’ expression, namely NANOG, OCT4 and SOX2. Immunofuorescence analysis suggested a general increase in the markers’ concentration in spheroids relative to the parental cells. RT-qPCR and immunoblots showed an increase in the stem-cell marker expression in spheroids generated from doxorubicin-treated when compared to non-treated cells. In spheroids, Sox2 was signifcantly upregulated in doxorubicintreated spheroids, whereas Nanog and Oct4 were generally downregulated when compared to non-treated spheroids. Both |
Keywords | Trophoblast; Stem-like cells; Doxorubicin |
Year | 2022 |
Journal | 3 Biotech |
Journal citation | 12 (184), pp. 1-11 |
Publisher | Springer |
ISSN | 2190-5738 |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s13205-022-03243-x |
Web address (URL) | https://link.springer.com/content/pdf/10.1007/s13205-022-03243-x.pdf |
Publisher's version | License File Access Level Open |
Output status | Published |
Publication dates | |
Online | 25 Jun 2022 |
Publication process dates | |
Accepted | 17 Jan 2022 |
Deposited | 04 Aug 2022 |
https://repository.derby.ac.uk/item/97yy7/doxorubicin-resistant-choriocarcinoma-cell-line-derived-spheroidal-cells-exhibit-stem-cell-markers-but-reduced-invasion
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Publisher's version
Balahmar2022_Article_DoxorubicinResistantChoriocarc (1).pdf | ||
License: CC BY 4.0 | ||
File access level: Open |
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