Characterization and phylogeny of fungi isolated from industrial wastewater using multiple genes

Journal article


Ezeonuegbu, B.A., Abdullahi, M.D., Whong, C.M., Sohunago, J.W., Kassem, H.S., Yaro, C.A., Hetta, H.F., Mostafa-Hedeab, G., Zouganelis, G. and Batiha, G.E.S 2022. Characterization and phylogeny of fungi isolated from industrial wastewater using multiple genes. Scientific Reports. 12, pp. 1-11. https://doi.org/10.1038/s41598-022-05820-9
AuthorsEzeonuegbu, B.A., Abdullahi, M.D., Whong, C.M., Sohunago, J.W., Kassem, H.S., Yaro, C.A., Hetta, H.F., Mostafa-Hedeab, G., Zouganelis, G. and Batiha, G.E.S
Abstract

The aim of this study was the isolation and molecular characterization of fungi from untreated refinery effluent by using multiple conserved genes. The Fungi isolated were characterized based on PCR amplification and genomic sequencing of the internal transcribed spacer region (ITS), partial β-tubulin (BenA), calmodulin (CaM), and RNA polymerase second large subunit (RPB2) genes, along with morphological characterization. The obtained sequences were subjected to BLAST analysis and the corresponding fungal isolates were assigned species names after comparison with representative sequences available in GenBank. Fifteen (15) Fungi species belonging to four genera of Aspergillus, Penicillium, Fusarium, and Trichoderma with Aspergillus as the predominant genus were identified. Therefore these genes should be used as molecular markers for species level identification of fungi (especially Aspergillus and Penicillium as proven in this study.

KeywordsFungi; Fungal Genes; PCR; Phylogeny
Year2022
JournalScientific Reports
Journal citation12, pp. 1-11
PublisherSpringer Nature
ISSN 2045-2322
Digital Object Identifier (DOI)https://doi.org/10.1038/s41598-022-05820-9
Web address (URL)https://www.nature.com/articles/s41598-022-05820-9
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Open
Output statusPublished
Publication dates
Online08 Feb 2022
Publication process dates
Deposited16 Sep 2022
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https://repository.derby.ac.uk/item/99279/characterization-and-phylogeny-of-fungi-isolated-from-industrial-wastewater-using-multiple-genes

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