Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP‐1 secretory activity in vitro and acute glucose lowering effects in mice
Journal article
Authors | Parthsarathy, Vadivel, Mclaughlin, Christopher, Harnedy, Padraigin, Allsopp, Phillip, Crowe, William, McSorley, Emeir, FitzGerald, Dick and O'Harte, Finbarr |
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Abstract | The anti‐diabetic actions of a boarfish protein hydrolysate (BPH) were investigated in cultured cells and mice. A boarfish (Capros aper) muscle protein hydrolysate was generated using the enzymes Alcalase 2.4 L and Flavourzyme 500 L. Furthermore, the BPH was subjected to simulated gastrointestinal digestion (SGID). BPH and SGID samples (0.01–2.5 mg mL−1) were tested in vitro for DPP‐IV inhibition and insulin and GLP‐1 secretory activity from BRIN‐BD11 and GLUTag cells, respectively. The BPH and SGID samples, caused a dose‐dependent increase (4.2 to 5.3‐fold, P < 0.001) in insulin secretion from BRIN‐BD11 cells and inhibited DPP‐IV activity (IC50 1.18 ± 0.04 and 1.21 ± 0.04 mg mL−1), respectively. The SGID sample produced a 1.3‐fold (P < 0.01) increase in GLP‐1 secretion. An oral glucose tolerance test (OGTT) was conducted in healthy mice (n = 8), with or without BPH (50 mg/kg bodyweight). BPH mediated an increase in plasma insulin levels (AUC(0–120 min), P < 0.05) and a consequent reduction in blood glucose concentration (P < 0.01), after OGTT in mice versus controls. The BPH showed potent anti‐diabetic actions in cells and improved glucose tolerance in mice. |
Keywords | Boarfish protein hydrolysate; dipeptidyl peptidase-IV inhibition; Glucagon-like peptide-1; glycaemic control in mice; insulin secretion |
Year | 2018 |
Journal | International Journal of Food Science and Technology |
Journal citation | 54 (1), pp. 271-281 |
Publisher | Wiley |
ISSN | 09505423 |
Digital Object Identifier (DOI) | https://doi.org/10.1111/ijfs.2019.54.issue-1 |
https://doi.org/10.1111/ijfs.13975 | |
Web address (URL) | http://europepmc.org/article/AGR/IND606268062 |
https://pure.ulster.ac.uk/en/publications/boarfish-capros-aper-protein-hydrolysate-has-potent-insulinotropi | |
http://hdl.handle.net/10545/624645 | |
hdl:10545/624645 | |
Output status | Published |
Publication dates | 16 Oct 2018 |
Publication process dates | |
Deposited | 01 Apr 2020 |
Accepted | 10 Sep 2018 |
Rights | CC0 1.0 Universal |
Contributors | University of Ulster and University of Limerick |
File | File Access Level Open |
File | File Access Level Open |
https://repository.derby.ac.uk/item/92665/boarfish-capros-aper-protein-hydrolysate-has-potent-insulinotropic-and-glp-1-secretory-activity-in-vitro-and-acute-glucose-lowering-effects-in-mice
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