The development of an eDNA based detection method for the invasive shrimp Dikerogammarus haemobaphes
Journal article
Authors | Mauvisseau, Quentin, Troth, Chris, Young, Emily, Burian, Alfred and Sweet, Michael |
---|---|
Abstract | Dikerogammarus haemobaphes is a freshwater gammarid crustacean native to the Ponto-Caspian region. However, the species is rapidly spreading throughout Western Europe and is classed as a highly invasive species. Here we present a novel eDNA assay aimed at detecting D. haemobaphes and demonstrate its suitability with validation steps conducted in-silico (computer simulations), ex-situ (test of specificity using closely related species) and in-situ (within the field). A survey of freshwater systems in the West-Midlands, United Kingdom, highlighted that D. haemobaphes was present in 26 out of the 39 sites assessed. We conclude that eDNA detection for D. haemobaphes is a promising tool for assessing and mapping the presence/distribution of this invasive amphipod. |
Keywords | invasive species; eDNA; limit of detection; early detection; freshwater systems; environmental DNA |
Year | 2019 |
Journal | Management of Biological Invasions |
Journal citation | 10 (3), pp. 449-460 |
Publisher | Regional Euro-Asian Biological Invasions Centre Oy (REABIC) |
ISSN | 1989-8649 |
Digital Object Identifier (DOI) | https://doi.org/10.3391/mbi.2019.10.3.03 |
Web address (URL) | https://www.reabic.net/journals/mbi/2019/Issue3.aspx |
hdl:10545/624403 | |
Output status | Published |
Publication dates | 06 Jun 2019 |
Publication process dates | |
Deposited | 15 Jan 2020, 16:14 |
Accepted | 19 Mar 2019 |
Contributors | University of Derby and Surescreen Scientifics Ltd, Morley |
File | File Access Level Restricted |
https://repository.derby.ac.uk/item/92vvw/the-development-of-an-edna-based-detection-method-for-the-invasive-shrimp-dikerogammarus-haemobaphes
368
total views0
total downloads8
views this month0
downloads this month