Study to investigate the prevalence of human papillomavirus in Barrett’s oesophagus using a novel screening methodology
Journal article
Authors | Marsh, E.K., White, J.R., Ragunath, K., Whitton, A., Kaye, P. and Knight, G.L. |
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Abstract | Human papillomavirus (HPV) is strongly associated with Barrett’s dysplasia and oesophageal cancer suggesting a role in carcinogenesis. HPV persistence predicts treatment failure after endotherapy for Barrett’s dysplasia. This pilot study applies a novel HPV screening tool (previously only used in the oropharynx) to detect HPV DNA directly and determine the prevalence rates in Barrett’s oesophagus (BO). DNA was extracted from 20 formalin-fixed BO samples. HPV DNA was detected using real-time PCR and gel electrophoresis. 5 out of 20 patients were identified as positive for HPV. Prevalence was 25% in patients with BO. This method can be used in BO’s tissue to determine HPV infection. Adoption of this as a screening test could potentially revolutionise future research in this area. If a clear link between HPV and Barrett’s dysplasia can be confirmed, this qPCR method has the potential to aid in monitoring and/or dysplasia detection by stratifying those most at risk and aid in the development of new therapies. |
Keywords | Human papillomavirus; Barrett’s dysplasia; oesophageal cancer |
Year | 2022 |
Journal | BMJ Open Gastroenterology |
Journal citation | 9, pp. 1-5 |
Publisher | BMJ Publishing Group Ltd. |
ISSN | 2054-4774 |
Digital Object Identifier (DOI) | https://doi.org/10.1136/bmjgast-2021-000840 |
Web address (URL) | https://bmjopengastro.bmj.com/content/bmjgast/9/1/e000840.full.pdf |
Publisher's version | License File Access Level Open |
Output status | Published |
Publication dates | |
Online | 04 Apr 2022 |
Publication process dates | |
Accepted | 25 Jan 2022 |
Deposited | 20 Jan 2023 |
https://repository.derby.ac.uk/item/9w3yy/study-to-investigate-the-prevalence-of-human-papillomavirus-in-barrett-s-oesophagus-using-a-novel-screening-methodology
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