Study to investigate the prevalence of human papillomavirus in Barrett’s oesophagus using a novel screening methodology

Journal article


Marsh, E.K., White, J.R., Ragunath, K., Whitton, A., Kaye, P. and Knight, G.L. 2022. Study to investigate the prevalence of human papillomavirus in Barrett’s oesophagus using a novel screening methodology. BMJ Open Gastroenterology. 9, pp. 1-5. https://doi.org/10.1136/bmjgast-2021-000840
AuthorsMarsh, E.K., White, J.R., Ragunath, K., Whitton, A., Kaye, P. and Knight, G.L.
Abstract

Human papillomavirus (HPV) is strongly associated with Barrett’s dysplasia and oesophageal cancer suggesting a role in carcinogenesis. HPV persistence predicts treatment failure after endotherapy for Barrett’s dysplasia. This pilot study applies a novel HPV screening tool (previously only used in the oropharynx) to detect HPV DNA directly and determine the prevalence rates in Barrett’s oesophagus (BO). DNA was extracted from 20 formalin-fixed BO samples. HPV DNA was detected using real-time PCR and gel electrophoresis. 5 out of 20 patients were identified as positive for HPV. Prevalence was 25% in patients with BO. This method can be used in BO’s tissue to determine HPV infection. Adoption of this as a screening test could potentially revolutionise future research in this area. If a clear link between HPV and Barrett’s dysplasia can be confirmed, this qPCR method has the potential to aid in monitoring and/or dysplasia detection by stratifying those most at risk and aid in the development of new therapies.

KeywordsHuman papillomavirus; Barrett’s dysplasia; oesophageal cancer
Year2022
JournalBMJ Open Gastroenterology
Journal citation9, pp. 1-5
PublisherBMJ Publishing Group Ltd.
ISSN2054-4774
Digital Object Identifier (DOI)https://doi.org/10.1136/bmjgast-2021-000840
Web address (URL)https://bmjopengastro.bmj.com/content/bmjgast/9/1/e000840.full.pdf
Publisher's version
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File Access Level
Open
Output statusPublished
Publication dates
Online04 Apr 2022
Publication process dates
Accepted25 Jan 2022
Deposited20 Jan 2023
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