Pellino-1 regulates the responses of the airway to viral infection
Journal article
Authors | Marsh, Elizabeth K, Prestwich, Elizabeth C, Marriott, Helen M, Williams, Lynne, Hart, Amber R, Muir, Claire F, Parker, Lisa C, Jonker, Marnix R, Heijink, Irene H, Timens, Wim, Fife, Mark, Hussell, Tracy, Hershenson, Marc B, Bentley, J Kelley, Sun, Shao-Cong, Barksby, Ben S, Borthwick, Lee A, Stewart, James P, Dockrell, David H and Sabroe, Ian |
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Abstract | Exposure to respiratory pathogens is a leading cause of exacerbations of airway diseases such as asthma and chronic obstructive pulmonary disease (COPD). Pellino-1 is an E3 ubiquitin ligase known to regulate virally-induced inflammation. We wished to determine the role of Pellino-1 in the host response to respiratory viruses in health and disease. Pellino-1 expression was examined in bronchial sections from patients with GOLD stage 2 COPD and healthy controls. Primary bronchial epithelial cells (PBECs), in which Pellino-1 expression had been knocked down, were extracellularly challenged with the TLR3 agonist poly(I:C). C57BL/6 Peli1-/- mice and wild type littermates were subjected to intranasal infection with clinically-relevant respiratory viruses; rhinovirus (RV1B) and influenza A. We find that Pellino-1 is expressed in the airways of normal subjects and those with COPD, and that Pellino-1 regulates TLR3 signalling and responses to airways viruses. In particular we observed that knockout of |
Keywords | Pellino-1, Influenza A virus, Rhinovirus, COPD, Asthma, Airway epithelia, Inflammation |
Year | 2020 |
Journal | Frontiers in Cellular and Infection Microbiology |
Publisher | Frontiers |
ISSN | 2235-2988 |
Digital Object Identifier (DOI) | https://doi.org/10.3389/fcimb.2020.00456/full |
Web address (URL) | http://hdl.handle.net/10545/625059 |
http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
hdl:10545/625059 | |
Publication dates | 31 Aug 2020 |
Publication process dates | |
Deposited | 27 Jul 2020, 09:26 |
Accepted | 24 Jul 2020 |
Rights | Attribution-NonCommercial-NoDerivatives 4.0 International |
Contributors | University of Derby, University of Sheffield, University of Groningen, University of Manchester, University of Michigan, University of Texas MD Anderson Cancer Center, Newcastle University, University of Liverpool and University of Edinburgh |
File | File Access Level Open |
File | File Access Level Open |
https://repository.derby.ac.uk/item/93684/pellino-1-regulates-the-responses-of-the-airway-to-viral-infection
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