Impact of one-step luminescent cyanoacrylate treatment on subsequent DNA analysis
Journal article
Authors | Alicia Khuu, Scott Chadwick, Moret, S., Xanthe Spindler, Peter Gunn and Claude Roux |
---|---|
Abstract | Fingermarks can be exploited for both their ridge detail and touch DNA. One-step luminescent cyanoacrylate (CA) fuming techniques used for fingermark enhancement, such as PolyCyano UV (Foster + Freeman Ltd) and Lumicyano™ (Crime Science Technology), claim to be compatible with DNA analysis as they reduce the need for post‐staining to increase contrast of the developed fingermark. The aim of this study was to determine the impact that these one-step luminescent cyanoacrylates have on DNA analysis and how they compare to conventional CA techniques. Four donors each deposited five sets of natural fingermarks, to which a known amount of washed saliva cells was dispensed onto half of each set of fingermarks. Each set was treated with either a conventional CA technique or a one‐step luminescent CA technique prior to collection and processing of DNA, with one set left as a non-fumed control. It was found that DNA was still recoverable and detectable following each of the treatments. Lumicyano™ had a similar impact on DNA profiles as conventional CA fuming and with post‐stain, however, the degradation effect of PolyCyano UV on DNA was greater than the conventional treatments. For quantities of DNA such as that from touch DNA, the use of PolyCyano UV to enhance fingermarks may impact subsequent DNA analysis by causing allele drop out at larger fragment sizes. |
Keywords | DNA degradation; Fingermark; Fingerprint; Lumicyano™; PolyCyano UV; Touch DNA |
Year | 2018 |
Journal | Forensic Science International |
Journal citation | Vol 286 (May2018), pp. 1-7 |
Publisher | Elseiver |
ISSN | 03790738 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.forsciint.2018.02.015 |
Web address (URL) | https://doi.org/10.1016/j.forsciint.2018.02.015 |
https://pubmed.ncbi.nlm.nih.gov/29524741/ | |
Output status | Published |
Publication dates | |
Online | 23 Feb 2018 |
Online | 11 Apr 2018 |
May 2018 | |
Publication process dates | |
Accepted | 15 Feb 2018 |
Deposited | 24 May 2023 |
https://repository.derby.ac.uk/item/9yy57/impact-of-one-step-luminescent-cyanoacrylate-treatment-on-subsequent-dna-analysis
43
total views0
total downloads3
views this month0
downloads this month