Surface functionalized perovskite nanocrystals: a design strategy for organelle-specific fluorescence lifetime multiplexing

Journal article

Anik Kumar Dey, Subhadeep Das, Sharon Mary Jose, Sreejesh Sreedharan, Noufal Kandoth, Surajit Barman, Abhijit Patra, Amitava Das and Sumit Kumar Pramanik 2024. Surface functionalized perovskite nanocrystals: a design strategy for organelle-specific fluorescence lifetime multiplexing. Chemical Science. pp. 1-10. https://doi.org/10.1039/d4sc01447b
AuthorsAnik Kumar Dey, Subhadeep Das, Sharon Mary Jose, Sreejesh Sreedharan, Noufal Kandoth, Surajit Barman, Abhijit Patra, Amitava Das and Sumit Kumar Pramanik
Abstract

Fluorescent molecules or materials with high photoluminescence quantum yields and stability towards photobleaching are ideally suited for multiplex imaging. Despite complying with such properties, perovskite nanocrystals (Pv-NCs) are rarely used for bioimaging owing to their toxicity and limited stability in aqueous media and towards human physiology. We aim to address these deficiencies by designing core–shell structures with Pv-NCs as the core and surface-engineered silica as the shell (SiO2@Pv-NCs) since silica is recognized as a biologically benign carrier material and is known to be excreted through urine. The post-grafting methodology is adopted for developing [SiO2@Pv-NCs]tpm and [SiO2@Pv-NCs]tsy (tpm: triphenylphosphonium ion, tsy: tosylsulfonamide) for specific imaging of mitochondria and endoplasmic reticulum (ER) of the live HeLa cell, respectively.

Keywordsperovskite nanocrystals; Fluorescent molecules; photoluminescence quantum yields
Year2024
JournalChemical Science
Journal citationpp. 1-10
PublisherRoyal Society of Chemistry
ISSN2041-6539
Digital Object Identifier (DOI)https://doi.org/10.1039/d4sc01447b
Web address (URL)https://doi.org/10.1039/D4SC01447B
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Output statusPublished
Publication dates
Online10 Jun 2024
2024
Publication process dates
Accepted06 Jun 2024
Deposited12 Jul 2024
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