Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe

Journal article

Ali, F., Aute, S., Sreedharan, S., Anila, H.A., Saeed, H.K., Smythe, C.G., Thomas, J.A. and Das, A. 2018. Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe. Chemical Communications. 54 (15), pp. 1849-185. https://doi.org/10.1039/c7cc09433g
AuthorsAli, F., Aute, S., Sreedharan, S., Anila, H.A., Saeed, H.K., Smythe, C.G., Thomas, J.A. and Das, A.
Abstract

BODIPY derivative, SF-1, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles. SF-1 is compatible with 3D-SIM imaging of individual cells.

KeywordsBODIPY derivative; Fluorescence ON response; Widefield microscopy
Year2018
JournalChemical Communications
Journal citation54 (15), pp. 1849-185
PublisherAmerican Chemical Society
ISSN1359-7345
1364-548X
Digital Object Identifier (DOI)https://doi.org/10.1039/c7cc09433g
Web address (URL)https://eprints.whiterose.ac.uk/127139/
http://dx.doi.org/10.1039/c7cc09433g
Output statusPublished
Publication dates31 Jan 2018
Publication process dates
Accepted25 Jan 2018
Deposited12 Jul 2024
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