Nanocarriers used as probes for super-resolution microscopy

Journal article

Sreejesh Sreedharan, Tiwari, S., Tyde, D., Aderinto, S. O., Pramanik, S. K., Das, A. and Thomas, J. A. 2020. Nanocarriers used as probes for super-resolution microscopy. Materials Chemistry Frontiers. 5 (3), pp. 1268-1282. https://doi.org/10.1039/d0qm00684j
AuthorsSreejesh Sreedharan, Tiwari, S., Tyde, D., Aderinto, S. O., Pramanik, S. K., Das, A. and Thomas, J. A.
Abstract

Super-resolution microscopy (SRM) has revolutionized the study of cell biology, enabling researchers to visualize cellular structures with nanometric resolution, single-molecule sensitivity, and with multiple colors using conventional fluorophores. SRM is well-suited for volumetric live-cell imaging and helps in extracting quantitative information on spatial distributions. It can be used to estimate the absolute numbers of proteins or other macromolecules or nanostructured material within subcellular compartments, characterize their structures, and their nano/bio interactions. Although a number of recent general reviews on SRM have elaborated its role in chemical and clinical biology, as well as nanomedicine, herein, we provide an overview of the use of luminescent nanocarriers (LNC) in SRM imaging and single-molecule tracking. The role of LNCs in controlling the brightness and stability of emissive states is discussed with a special focus on organelle-specific delivery and how this approach can be utilized to produce novel optical-switched systems. We also discuss the challenges related to the molecular targeting of such material in biological systems. In doing so, we will provide practical guidance for super-resolution imaging in nanomedicine research, its technical challenges, and the opportunities for future advancement.

KeywordsSuper-resolution microscopy (SRM); cell biology; conventional fluorophores; nanomedicine
Year2020
JournalMaterials Chemistry Frontiers
Journal citation5 (3), pp. 1268-1282
PublisherAmerican Chemical Society
ISSN2052-1537
Digital Object Identifier (DOI)https://doi.org/10.1039/d0qm00684j
Web address (URL)https://doi.org/10.1039/D0QM00684J
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Output statusPublished
Publication dates10 Nov 2020
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Deposited12 Jul 2024
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