Multimodal Super-resolution Optical Microscopy Using a Transition-Metal-Based Probe Provides Unprecedented Capabilities for Imaging Both Nuclear Chromatin and Mitochondria

Journal article

Sreedharan, S., Gill, M.R., Garcia, E., Saeed, H.K., Robinson, D., Byrne, A., Cadby, A., Keyes, T.E., Smythe, C., Pellett, P. and Bernardino de la Serna, J. 2017. Multimodal Super-resolution Optical Microscopy Using a Transition-Metal-Based Probe Provides Unprecedented Capabilities for Imaging Both Nuclear Chromatin and Mitochondria. Journal of the American Chemical Society. 139 (4), pp. 15907-15913. https://doi.org/10.1021/jacs.7b08772
AuthorsSreedharan, S., Gill, M.R., Garcia, E., Saeed, H.K., Robinson, D., Byrne, A., Cadby, A., Keyes, T.E., Smythe, C., Pellett, P. and Bernardino de la Serna, J.
Abstract

Detailed studies on the live cell uptake properties of a dinuclear membrane-permeable RuII cell probe show that, at low concentrations, the complex localizes and images mitochondria. At concentrations above ∼20 μM, the complex images nuclear DNA. Because the complex is extremely photostable, has a large Stokes shift, and displays intrinsic subcellular targeting, its compatibility with super-resolution techniques was investigated. It was found to be very well suited to image mitochondria and nuclear chromatin in two color, 2C-SIM, and STED and 3D-STED, both in fixed and live cells. In particular, due to its vastly improved photostability compared to that of conventional SR probes, it can provide images of nuclear DNA at unprecedented resolution.

KeywordsDinuclear RuII cell probe; Membrane-permeable; Live cell uptake
Year2017
JournalJournal of the American Chemical Society
Journal citation139 (4), pp. 15907-15913
PublisherAmerican Chemical Society
ISSN0002-7863
1520-5126
Digital Object Identifier (DOI)https://doi.org/10.1021/jacs.7b08772
Web address (URL)https://eprints.whiterose.ac.uk/122534/
http://dx.doi.org/10.1021/jacs.7b08772
Output statusPublished
Publication dates04 Oct 2017
Publication process dates
Deposited12 Jul 2024
Permalink -

https://repository.derby.ac.uk/item/q7516/multimodal-super-resolution-optical-microscopy-using-a-transition-metal-based-probe-provides-unprecedented-capabilities-for-imaging-both-nuclear-chromatin-and-mitochondria

Log in to edit
  • 15
    total views
  • 0
    total downloads
  • 0
    views this month
  • 0
    downloads this month

Export as

Related outputs

Surface functionalized perovskite nanocrystals: a design strategy for organelle-specific fluorescence lifetime multiplexing
Anik Kumar Dey, Subhadeep Das, Sharon Mary Jose, Sreejesh Sreedharan, Noufal Kandoth, Surajit Barman, Abhijit Patra, Amitava Das and Sumit Kumar Pramanik 2024. Surface functionalized perovskite nanocrystals: a design strategy for organelle-specific fluorescence lifetime multiplexing. Chemical Science. pp. 1-10. https://doi.org/10.1039/d4sc01447b
From Chemotherapy to Phototherapy – Changing the Therapeutic Action of a Metallo-Intercalating RuII-ReI Luminescent System by Switching its Sub-Cellular Location
Saeed, H.K.,, Jarman, P.J., Sreedharan, S., Mowll, R., Auty, A.J., Chauvet, A.A., Smythe, C.G., de la Serna, J.B. and Thomas, J.A. 2023. From Chemotherapy to Phototherapy – Changing the Therapeutic Action of a Metallo-Intercalating RuII-ReI Luminescent System by Switching its Sub-Cellular Location. Chemistry: A European Journal . 29 (34), pp. 1-6. https://doi.org/10.1002/chem.202300617
Nanomaterial-induced toxicity in pathophysiological models representative of individuals with pre-existing medical conditions
Sreejesh Sreedharan, Zouganelis, G., Samantha J Drake, Gyanendra Tripathi and Ali Kermanizadeh 2022. Nanomaterial-induced toxicity in pathophysiological models representative of individuals with pre-existing medical conditions. Journal of Toxicology and Environmental Health, Part B: Critical Reviews. pp. 1-27. https://doi.org/10.1080/10937404.2022.2153456
Nanoparticles for super-resolution microscopy: intracellular delivery and molecular targeting
Sumit Kumar Pramanik, Sreejesh Sreedharan, Rajeshwari Tiwari, Sourav Dutta, Noufal Kandoth, Surajit Barman, Stephen O Aderinto, Samit Chattopadhyay, Amitava Das and Jim A Thomas 2022. Nanoparticles for super-resolution microscopy: intracellular delivery and molecular targeting. Chemical Society Reviews. 51, pp. 9882-9916. https://doi.org/10.1039/d1cs00605c
Ultrasensitive Reagent for Ratiometric Detection and Detoxification of iAsIII in Water and Mitochondria
Sheik Saleem Pasha, Amrita Banerjee, Sreejesh Sreedharan, Soumendra Singh, Noufal Kandoth, Katherine A. Vallis, Samir Kumar Pal, Sumit Kumar Pramanik and Amitava Das 2022. Ultrasensitive Reagent for Ratiometric Detection and Detoxification of iAsIII in Water and Mitochondria. Inorganic Chemistry. 61 (33), p. 13115–13124. https://doi.org/10.1021/acs.inorgchem.2c01761
A Dinuclear Osmium(II) Complex Near-Infrared Nanoscopy Probe for Nuclear DNA
Dröge, F., Noakes, F.F., Archer, S.A., Sreedharan, S., Raza, A., Robertson, C.C., MacNeil, S., Haycock, J.W., Carson, H., Meijer, A.J. and Smythe, C.G. 2021. A Dinuclear Osmium(II) Complex Near-Infrared Nanoscopy Probe for Nuclear DNA. Journal of the American Chemical Society. 143 (48), p. 20442–20453. https://doi.org/10.1021/jacs.1c10325
Photoactive metal complexes that bind DNA and other biomolecules as cell probes, therapeutics, and theranostics
Saeed, H. K., Sreejesh Sreedharan and Thomas, J. A. 2020. Photoactive metal complexes that bind DNA and other biomolecules as cell probes, therapeutics, and theranostics. Chemical Communications. 56 (10), pp. 1464-80. https://doi.org/10.1039/c9cc09312e
Making the Right Link to Theranostics: The Photophysical and Biological Properties of Dinuclear RuII–ReI dppz Complexes Depend on Their Tether
Hiwa K. Saeed, Sreejesh Sreedharan, Paul J. Jarman, Stuart A. Archer, Simon D. Fairbanks, Simon P. Foxon, Alexander J. Auty, Dimitri Chekulaev, Theo Keane, Anthony J. H. M. Meijer, Julia A. Weinstein, Carl G. W. Smythe, Jorge Bernardino de la Serna and Jim A. Thomas 2020. Making the Right Link to Theranostics: The Photophysical and Biological Properties of Dinuclear RuII–ReI dppz Complexes Depend on Their Tether. Journal of the American Chemical Society. 142 (2), p. 1101–1111. https://doi.org/10.1021/jacs.9b12564
Nanocarriers used as probes for super-resolution microscopy
Sreejesh Sreedharan, Tiwari, S., Tyde, D., Aderinto, S. O., Pramanik, S. K., Das, A. and Thomas, J. A. 2020. Nanocarriers used as probes for super-resolution microscopy. Materials Chemistry Frontiers. 5 (3), pp. 1268-1282. https://doi.org/10.1039/d0qm00684j
Mitochondriotropic lanthanide nanorods: implications for multimodal imaging
Singh, H., Sreejesh Sreedharan, Oyarzabal, E., Mahapatra, T. S., Green, N., Ian Shih, Y., Das, M., Thomas, J. A., Pramanik, S. K. and Das, A. 2020. Mitochondriotropic lanthanide nanorods: implications for multimodal imaging. Chemical Communications. 57, pp. 1-11. https://doi.org/10.1039/d0cc02698k
Photoactivable Prodrug for Simultaneous Release of Mertansine, and CO along with a BODIPY Derivative as a Luminescent Marker in Mitochondria: A Proof of Concept for NIR Image-Guided Cancer Therapy
Tiwari, R., Shinde, P.S., Sreedharan, S., Dey, A.K., Vallis, K.A., Mhaske, S.B., Pramanik, S.K. and Das, A 2020. Photoactivable Prodrug for Simultaneous Release of Mertansine, and CO along with a BODIPY Derivative as a Luminescent Marker in Mitochondria: A Proof of Concept for NIR Image-Guided Cancer Therapy. Chemical Science. 12 (7), pp. 2667-2673. https://doi.org/10.1039/d0sc06270g
Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe
Ali, F., Aute, S., Sreedharan, S., Anila, H.A., Saeed, H.K., Smythe, C.G., Thomas, J.A. and Das, A. 2018. Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe. Chemical Communications. 54 (15), pp. 1849-185. https://doi.org/10.1039/c7cc09433g
Polysulfide-triggered fluorescent indicator suitable for super-resolution microscopy and application in imaging
Sreedharan, S., Hoskere, A., Ali, F., Smythe, G.C., Thomas, J. A. and Das, A. 2018. Polysulfide-triggered fluorescent indicator suitable for super-resolution microscopy and application in imaging. Chemical Communications. 54 (30), pp. 3735-3738. https://doi.org/10.1039/c8cc01332b
Mitochondria-localising DNA-binding biscyclometalated phenyltriazole iridium(iii) dipyridophenazene complexes: syntheses and cellular imaging properties
Sinopoli, A., Sreedharan, S., Jarman, P.J., Robinson, D., Clemmet, C., Scattergood, P.A., Rice, C.R., Smythe, C.G., Thomas, J.A. and Elliott, P.I. 2018. Mitochondria-localising DNA-binding biscyclometalated phenyltriazole iridium(iii) dipyridophenazene complexes: syntheses and cellular imaging properties. Dalton Transactions. 47 (14), pp. 4931-4940. https://doi.org/10.1039/c8dt00046h
A Fluorescent Chemodosimeter for Organelle-Specific Imaging of Nucleoside Polyphosphate Dynamics in Living Cells
Singh, H., Sreedharan, S., Tiwari, R., Walther, C., Smythe, C., Pramanik, S.K., Thomas, J.A. and Das, A. 2018. A Fluorescent Chemodosimeter for Organelle-Specific Imaging of Nucleoside Polyphosphate Dynamics in Living Cells. Crystal Growth & Design. 18 (11), pp. 7199-7206. https://doi.org/10.1021/acs.cgd.8b01409
Two photon excitable graphene quantum dots for structured illumination microscopy and imaging applications: lysosome specificity and tissue-dependent imaging
Singh, H., Sreedharan, S., Tiwari, K., Green, N.H., Smythe, C., Pramanik, S.K., Thomas, J.A. and Das, A. 2018. Two photon excitable graphene quantum dots for structured illumination microscopy and imaging applications: lysosome specificity and tissue-dependent imaging. Chemical Communications. 55 (4), pp. 521-524. https://doi.org/10.1039/c8cc08610a
Mitochondria Targeting Non-Isocyanate-Based Polyurethane Nanocapsules for Enzyme-Triggered Drug Release
Pramanik, S.K., Sreedharan, S., Singh, H., Khan, M., Tiwari, K., Shiras, A., Smythe, C., Thomas, J.A. and Das, A. 2018. Mitochondria Targeting Non-Isocyanate-Based Polyurethane Nanocapsules for Enzyme-Triggered Drug Release. Bioconjugate Chemistry. 29 (11), pp. 3532-3543. https://doi.org/10.1021/acs.bioconjchem.8b00460
Imaging cellular trafficking processes in real time using lysosome targeted up-conversion nanoparticles
Pramanik, S.K., Sreedharan, S., Singh, H., Green, N.H., Smythe, C., Thomas, J.A. and Das, A. 2017. Imaging cellular trafficking processes in real time using lysosome targeted up-conversion nanoparticles. Chemical Communications. 53 (94), pp. 12672-12675. https://doi.org/10.1039/c7cc08185e
A Super-Resolution Probe To Monitor HNO Levels in the Endoplasmic Reticulum of Cells
Ali, F., Sreedharan, S., Ashoka, A.H., Saeed, H.K., Smythe, C.G., Thomas, J.A. and Das, A. 2017. A Super-Resolution Probe To Monitor HNO Levels in the Endoplasmic Reticulum of Cells. Analytical Chemistry. 89 (22), pp. 12087-12093. https://doi.org/10.1021/acs.analchem.7b02567
Homo- and Heteroleptic Phototoxic Dinuclear Metallo-Intercalators Based on RuII(dppn) Intercalating Moieties: Synthesis, Optical, and Biological Studies
Saeed, H.K., Jarman, P.J., Archer, S., Sreedharan, S., Saeed, I.Q., Mckenzie, L.K., Weinstein, J.A., Buurma, N.J., Smythe, C.G. and Thomas, J.A. 2017. Homo- and Heteroleptic Phototoxic Dinuclear Metallo-Intercalators Based on RuII(dppn) Intercalating Moieties: Synthesis, Optical, and Biological Studies. Angewandte Chemie. 56 (41), pp. 12628-12633. https://doi.org/10.1002/ange.201707350
Terminal PEGylated DNA–Gold Nanoparticle Conjugates Offering High Resistance to Nuclease Degradation and Efficient Intracellular Delivery of DNA Binding Agents
Song, L., Guo, Y., Roebuck, D., Chen, C., Yang, M., Yang, Z., Sreedharan, S., Glover, C., Thomas, J.A., Liu, D. and Guo, S. Terminal PEGylated DNA–Gold Nanoparticle Conjugates Offering High Resistance to Nuclease Degradation and Efficient Intracellular Delivery of DNA Binding Agents. ACS Applied Materials & Interfaces. 7 (33), pp. 18707-18716. https://doi.org/10.1021/acsami.5b05228