Multimodal Super-resolution Optical Microscopy Using a Transition-Metal-Based Probe Provides Unprecedented Capabilities for Imaging Both Nuclear Chromatin and Mitochondria

Journal article

Sreedharan, S., Gill, M.R., Garcia, E., Saeed, H.K., Robinson, D., Byrne, A., Cadby, A., Keyes, T.E., Smythe, C., Pellett, P. and Bernardino de la Serna, J. 2017. Multimodal Super-resolution Optical Microscopy Using a Transition-Metal-Based Probe Provides Unprecedented Capabilities for Imaging Both Nuclear Chromatin and Mitochondria. Journal of the American Chemical Society. 139 (4), pp. 15907-15913. https://doi.org/10.1021/jacs.7b08772
AuthorsSreedharan, S., Gill, M.R., Garcia, E., Saeed, H.K., Robinson, D., Byrne, A., Cadby, A., Keyes, T.E., Smythe, C., Pellett, P. and Bernardino de la Serna, J.
Abstract

Detailed studies on the live cell uptake properties of a dinuclear membrane-permeable RuII cell probe show that, at low concentrations, the complex localizes and images mitochondria. At concentrations above ∼20 μM, the complex images nuclear DNA. Because the complex is extremely photostable, has a large Stokes shift, and displays intrinsic subcellular targeting, its compatibility with super-resolution techniques was investigated. It was found to be very well suited to image mitochondria and nuclear chromatin in two color, 2C-SIM, and STED and 3D-STED, both in fixed and live cells. In particular, due to its vastly improved photostability compared to that of conventional SR probes, it can provide images of nuclear DNA at unprecedented resolution.

KeywordsDinuclear RuII cell probe; Membrane-permeable; Live cell uptake
Year2017
JournalJournal of the American Chemical Society
Journal citation139 (4), pp. 15907-15913
PublisherAmerican Chemical Society
ISSN0002-7863
1520-5126
Digital Object Identifier (DOI)https://doi.org/10.1021/jacs.7b08772
Web address (URL)https://eprints.whiterose.ac.uk/122534/
http://dx.doi.org/10.1021/jacs.7b08772
Output statusPublished
Publication dates04 Oct 2017
Publication process dates
Deposited12 Jul 2024
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