A Fluorescent Chemodosimeter for Organelle-Specific Imaging of Nucleoside Polyphosphate Dynamics in Living Cells

Journal article

Singh, H., Sreedharan, S., Tiwari, R., Walther, C., Smythe, C., Pramanik, S.K., Thomas, J.A. and Das, A. 2018. A Fluorescent Chemodosimeter for Organelle-Specific Imaging of Nucleoside Polyphosphate Dynamics in Living Cells. Crystal Growth & Design. 18 (11), pp. 7199-7206. https://doi.org/10.1021/acs.cgd.8b01409
AuthorsSingh, H., Sreedharan, S., Tiwari, R., Walther, C., Smythe, C., Pramanik, S.K., Thomas, J.A. and Das, A.
Abstract

Nucleoside polyphosphates (NPPs) are mainly produced in mitochondria and used as a universal energy source for various cellular events. Although numerous fluorescent probes for adenosine triphosphate (ATP) have been reported, they are not ideally suited for live monitoring of the subtle variation of the mitochondrial ATP level. A new coumarin-based fluorescent probe is synthesized, and this reagent is utilized for specific recognition of NPPs in mitochondria by super-resolution microscopy in physiological condition. Detailed 31P NMR studies reveal that the probe, L·Zn(II), binds to NPPs through their pendent phosphate functionalities. Such binding leads to a substantial enhancement in the luminescence intensity of L·Zn(II) + ADP or L·Zn(II) + ATP as compared to L·Zn(II). This—as well as 1H NMR spectroscopy—has enabled us to evaluate the probe’s binding affinities to these NPPs. Structured illumination and wide field fluorescence microscopy confirmed that this physiologically benign reagent is localized within mitochondria of live RAW 264.7 macrophage cells. This reagent has been utilized to probe real-time changes in ATP concentrations within mitochondria during drug-induced apoptosis.

KeywordsNucleoside polyphosphates (NPPs) ; Mitochondria; Immunology; Luminescence
Year2018
JournalCrystal Growth & Design
Journal citation18 (11), pp. 7199-7206
PublisherAmerican Chemical Society
ISSN1528-7483
1528-7505
Digital Object Identifier (DOI)https://doi.org/10.1021/acs.cgd.8b01409
Web address (URL)https://pubs.acs.org/doi/10.1021/acs.cgd.8b01409
Output statusPublished
Publication dates08 Oct 2018
Publication process dates
Deposited12 Jul 2024
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