A Super-Resolution Probe To Monitor HNO Levels in the Endoplasmic Reticulum of Cells

Journal article

Ali, F., Sreedharan, S., Ashoka, A.H., Saeed, H.K., Smythe, C.G., Thomas, J.A. and Das, A. 2017. A Super-Resolution Probe To Monitor HNO Levels in the Endoplasmic Reticulum of Cells. Analytical Chemistry. 89 (22), pp. 12087-12093. https://doi.org/10.1021/acs.analchem.7b02567
AuthorsAli, F., Sreedharan, S., Ashoka, A.H., Saeed, H.K., Smythe, C.G., Thomas, J.A. and Das, A.
Abstract

Selective detection of nitroxyl (HNO), which has recently been identified as a reactive nitrogen species, is a challenging task. We report a BODIPY-based luminescence ON reagent for detection of HNO in aqueous solution and in live RAW 264.7 cells, based on the soft nucleophilicity of the phosphine oxide functionality toward HNO. The probe shows high selectivity to HNO over other reactive oxygen/nitrogen and sulfur species. Luminescence properties of the BODIPY-based chemodosimetric reagent make it an ideal candidate for use as a reagent for super-resolution structured illumination microscopy. The viability of the reagent for biological in vivo imaging application was also confirmed using Artemia as a model.

KeywordsSelective detection of nitroxyl (HNO); Biological imaging; Lasers; Peptides and proteins; Probes; Reagents
Year2017
JournalAnalytical Chemistry
Journal citation89 (22), pp. 12087-12093
PublisherAmerican Chemical Society
ISSN0003-2700
1520-6882
Digital Object Identifier (DOI)https://doi.org/10.1021/acs.analchem.7b02567
Web address (URL)https://eprints.whiterose.ac.uk/123245/
http://dx.doi.org/10.1021/acs.analchem.7b02567
Output statusPublished
Publication dates26 Oct 2017
Publication process dates
Deposited12 Jul 2024
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