Nanomaterial-induced cell death in pulmonary and hepatic cells following exposure to three different metallic materials: The role of autophagy and apoptosis

Journal article


Kermanizadeh, A., Jantzen, K., Ward, M.B., Durhuus, J.A., Juel Rasmussen, L., Loft, S. and Møller, P. 2017. Nanomaterial-induced cell death in pulmonary and hepatic cells following exposure to three different metallic materials: The role of autophagy and apoptosis. Nanotoxicology. Vol 11 (Issue 2), pp. 184-200. https://doi.org/10.1080/17435390.2017.1279359
AuthorsKermanizadeh, A., Jantzen, K., Ward, M.B., Durhuus, J.A., Juel Rasmussen, L., Loft, S. and Møller, P.
Abstract

Autophagy is the catabolic process involving the sequestration of the cytoplasm within double-membrane vesicles, which fuse with lysosomes to form autolysosomes in which autophagic targets are degraded. Since most endocytic routes of nanomaterial uptake converge upon the lysosome and the possibility that autophagy induction by NMs may be an attempt by the cell to self-preserve following the external challenge, this study investigated the role of autophagy following exposure to a panel of widely used metal-based NMs with high toxicity (Ag and ZnO) or low toxicity (TiO2) in a pulmonary (A549) and hepatic (HepG2) cell line. The in vitro exposure to the Ag and ZnO NMs resulted in the induction of both apoptosis and autophagy pathways in both cell types. However, the progression of autophagy was blocked in the formation of the autolysosome, which coincided with morphologic changes in the actin cytoskeleton. This response was not observed following the exposure to low-toxicity TiO2 NMs. Overall, the results show that high toxicity NMs can cause a dysfunction in the autophagy pathway which is associated with apoptotic cell death.

Keywordsapoptosis; autophagy; cell death; hepatic cells; Nanomaterials; pulmonary cells
Year2017
JournalNanotoxicology
Journal citationVol 11 (Issue 2), pp. 184-200
PublisherTaylor & Francis
ISSN1743-5404
1743-5390
Digital Object Identifier (DOI)https://doi.org/10.1080/17435390.2017.1279359
Web address (URL)http://www.scopus.com/inward/record.url?eid=2-s2.0-85010702305&partnerID=MN8TOARS
Output statusPublished
Publication dates
Online05 Jan 2017
Online24 Jan 2017
Online07 Feb 2017
Publication process dates
Deposited12 Jun 2023
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https://repository.derby.ac.uk/item/9z375/nanomaterial-induced-cell-death-in-pulmonary-and-hepatic-cells-following-exposure-to-three-different-metallic-materials-the-role-of-autophagy-and-apoptosis

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